Bioinformatics & Computational Biology (Module 1)

Fundamentals of bioinformatics (Introduction, origin, history & scope of bioinformatics), Importance & uses of bioinformatics and application of bioinformatics), Conceptual understanding of structural bioinformatics & molecular bioinformatics, Major databases in bioinformatics, Sequence database similarity searching tools: FASTA, BLAST (BLASTP, BLASTN, BLASTX, TBLASTX, TBLASTN), Primer Designing (Theory + Practical), Primary & secondary analysis of protein, Physical & chemical parameter analysis of protein, Detailed understanding of Homology Modelling, Concept of Molecular Docking (Drug Designing), Concept of MD Simulation, software & tools of MD simulation, Concept of sequencing: (Genome sequencing & Transcriptome sequencing), Detailed understanding of NGS analysis: Concept of Quality Control, Concept of Alignment & Assembly, Concept of DEGs analysis & Tools of NGS analysis. (Available at- NOIDA, Ahmedabad, Mumbai, Chandigarh, Chennai, Hyderabad, Jaipur, Dehradun and Gwalior)

Mammalian cell culture (Module 2)

Introduction to Cell culture/Mammalian cell culture/ Stem cell culture. Overview of cell culture for vaccine production, therapeutic proteins, gene therapy and cancer research, Biosafety recommendation on the handling of Mammalian cell cultures, Primary Cell culture and its maintenance, Contamination in cell culture, Basics of Adherent vs Suspension culture, Trypsinization, passaging for suspension culture (Shake off and scraping), Sterilization of all chemicals and instruments, Collection of blood samples and Cryopreservation, Isolation of Peripheral Blood Mononuclear Cells (PBMC) by sucrose gradient centrifuge, Preparation of PBMC suspension, Washing of PBMC isolates, Primary culture of PBMC into cell culture media, Handling and maintenance of CO2 incubator, Total viable cell counts through hemocytometer, Subculture of PBMC into culture media, Cell viability assay using MTT/alamar blue, Basic concept of Labelling, Culture and Handling of mammalian cell culture and its maintenance.

(Available at- NOIDA, Mumbai, Chandigarh and Hyderabad).

Cell and Molecular Biology (Module 3)

Introduction and scope of Cell and Molecular Biology, General and safety rules for working in the Lab, Demonstration of module-oriented instruments, Basic concept of percentage, molarity and normality, Principle-SOP and application of spectrophotometer, Composition of buffers (TAE and TBE). Overview of differential leukocyte cells, Preparation of blood smear and staining of blood cells, Genomic DNA/RNA isolation from plants, Genomic DNA/RNA isolation from bacteria. Basic concept for preparation of Tank buffer (TBE/TAE) - 6X loading dye and ETBr solution, Introduction and application of electrophoresis, Genomic DNA isolation from Hair/Nail/Blood, Qualitative analysis of DNA/RNA through Agarose Gel Electrophoresis. Genomic DNA quantification & purity check through UV-VIS spectrophotometer, Basic concept of PCR, Development of PCR assay, Performance of PCR in thermal cycler, Preparation of product labels, Types of PCR (RT-PCR, Gradient PCR, Nested PCR, and its application) (T).

(Available at- NOIDA, Ahmedabad, Mumbai, Chandigarh, Chennai, Hyderabad, Jaipur, Dehradun and Gwalior)

Medical and Clinical Microbiology (Module 4)

Introduction of medical and clinical microbiology, Overview and types of Sterilization techniques, Basic principle-Standard Operating Procedure (SOP)-application of instruments (Autoclave, pH meter, Laminar air flow, incubator, microscope and colony counter), Classification of bacteria based on Gram staining, Concept of basic calculation and safety rules for working in the Lab, Knowledge about BSL Labs and Classification, Types of media and solution preparation, Preparation and sterilization of selective media (EMB/ MSA/ MacConkey agar etc.), Isolation of pathogenic bacteria (Salmonella sp. Staphylococcus sp. Enterococcus sp. Proteus sp., E. coli, etc), Inoculation of pathogenic bacteria into nutrient broth medium, Different methods for the detection of antimicrobial activity, Basic concept of positive and negative controls, Antimicrobial activity test against isolated pathogenic bacteria by disc diffusion method, Minimum inhibitory concentration (MIC) via Agar diffusion method, Minimum bactericidal concentration (MBC) through Broth dilution method.

(Available at- NOIDA, Ahmedabad, Mumbai, Chandigarh, Chennai, Hyderabad, Jaipur, Dehradun and Gwalior)

Genomics & Recombinant DNA Technology (Module 5)

Introduction of Recombinant DNA Technology (RDT), Application of RDT, Recent trends and future perspectives in RDT, Good laboratory practices (GLP), Basic instrument handling, Concept of basic calculation (Percentage, Molarity and Normality), Micropipette handling (forward and reverse pipetting techniques), Competent cell preparation, Introduction about ligation, T4 DNA Ligation of λ DNA by HindIII restriction enzyme, Overview of Agarose gel electrophoresis, Preparation of Tank buffer (TBE/TAE) - 6X loading dye and ETBr solution. Observe the efficiency of the ligation reaction through Agarose gel electrophoresis, Overview of Transformation, Transformation of ligated DNA, Learn restriction-digestion technique (Plasmid DNA cut with EcoR1 and HindIII), Agarose Gel Electrophoresis of digested plasmid DNA, Basic concept of DNA fingerprinting by restriction fragment length polymorphism (RFLP) and Blue-white screening.

. (Available at- NOIDA, Ahmedabad, Mumbai, Chandigarh, Chennai, Hyderabad, Jaipur, Dehradun and Gwalior)

Proteomics (Module 6)

Introduction and application of proteomics, Good Laboratory Practices (GLP), Basic instrument handling, Concept of basic calculation (percentage, molarity, and normality), Reagent preparation and Micropipette handling (forward and reverse pipetting techniques), Different basic tests for protein analysis, Screening of protein-rich sources for protein estimation, Principle-Standard operating procedure (SOP) and application of spectrophotometer, Estimation of protein through UV-visible spectroscopy, Standard curve preparation and Data Interpretation. Extraction of protein from different sources, Different methods of protein precipitation, Basic concept of reagents preparation for SDS-PAGE- Stacking gel - running gel - sample loading dye- tank buffer- Staining solution - destaining solution, Protein separation through vertical gel electrophoresis (SDS-PAGE) technique, Concept of staining and destaining technique in SDS-PAGE, Staining and distaining of SDS-PAGE gel and observation, Overview of protein analysis by Western blotting technique and the difference between SDS-PAGE, Native PAGE and 2D PAGE (T).

(Available at- NOIDA, Ahmedabad, Mumbai, Chandigarh, Hyderabad, Jaipur, Dehradun and Gwalior)

Diabetes Single Nucleotide Polymorphism (SNP) Mutation Analysis (Module 7)

Introduction and scope of Molecular Biology, Overview of genomic DNA extraction, Bio-safety rules for working in a clinical lab, Demonstration of module-oriented instruments, Basic concept of molecular biology mathematics, Overview of Applied Molecular Biology, Basic handling and disposal of potential infectious/ clinical waste, Genomic DNA extraction from Blood, DNA sample quantification and qualitative analysis, Difference between Single Nucleotide Polymorphism and Mutations (Basic concept), Genetic risk factors for Diabetes and Molecular Diagnostics, SNP Polymerase Chain Reaction (SNP-PCR), SNP-PCR Amplicon gel electrophoresis, Data compilation, recording and interpretation, SNP analysis: Programs for prediction of Single Nucleotide Polymorphism effects.

(Available at- NOIDA, Ahmedabad, Mumbai, Hyderabad, Jaipur and Dehradun)

Enzyme and Microbial Technology (Module 8)

Introduction and application of enzymology, Enzyme market size & major companies in India, Good Laboratory Practices (GLP), Basic instrument handling, Micropipette handling (forward or reverse pipetting techniques). Concept of basic calculation of percentage, molarity, and normality, Classification of media and solution preparation, Principle-standard operation procedure and application of instruments (pH meter, autoclave, colony counter, and laminar airflow), Screening of microbes producing extracellular hydrolytic enzymes (Amylase/Protease/Lipase/Tannase etc.) from different source (soil/water/industrial effluent etc).Subculturing of enzyme producing bacteria on culture media, Preparation of seed culture for fermentation media, Overview of fermentation and amylase enzyme, Preparation of fermentation media for amylase enzyme, Production of enzyme using submerged fermentation method (for amylase enzyme).Extraction of crude amylase enzyme, Determination of amylase enzyme activity using biochemical test, Enzyme/cell immobilization technique through sodium alginate and calcium chloride method.

(Available at- NOIDA, Ahmedabad, Mumbai, Chandigarh, Chennai, Hyderabad, Jaipur, Dehradun and Gwalior)

Food Microbiology  (Module 9)

Introduction to food microbiology and Microbial Limit Test (MLT), Good laboratory practices (GLP), Basic instrument handling and micropipette handling (forward and reverse pipetting techniques), Application and calibration/validation (Autoclave, pH Meter, Laminar Air Flow, Colony Counter, Incubator, and Microscope), Review of microbiological logbook entries, Concept of basic calculation (percentage, molarity, and normality), Types of media and solution preparation, Overview and types of sterilization techniques, Classification of bacteria based on Gram staining, Preparation of solid media for Diarrhea-causing bacteria, Screening of Diarrhea-causing bacteria on selective media from spoiled food samples, Introduction about streaking and subculturing, Streaking technique used for isolation of bacteria. Characterization of Diarrhea causing bacteria by staining technique, Basics of biochemical test, Identification of isolates using different biochemical test (Indole test/ Citrate test/ Urease test etc.), Antimicrobial susceptibility test against Diarrhea causing bacteria, Methylene Blue Reductase Test (MBRT) in milk.

(Available at- NOIDA, Ahmedabad, Mumbai, Chandigarh, Chennai, Hyderabad, Jaipur, Dehradun and Gwalior)

Biochemistry & Immunology (Module 10)

Introduction to Biochemistry, Difference between antigen-antibody, Types of antibodies and basic instrument handling, Calculation (Percentage, Molarity and Normality), Micropipette handling (Reverse and Forward pipetting), Standard operating procedure (SOP) and application of different instruments (pH meter and spectrophotometer), Measurement of рН of biological liquids with universal indicator paper, Qualitative determination of Vitamin (A/C/D), Biuretic reaction for detection of protein, Qualitative test for the presence of carbohydrates (Molisch/ Tollen’s/ Fehling test), Quantitative determination of vitamin Р in the different samples via UV-VIS Spectrophotometer, Determination of alpha-Amylase activity (Serum/Saliva) on Starch, Components of blood. Bleeding time calculation, Clotting time estimation. Separation of Serum from human blood, Basic concept of antigen and antibody, Overview of enzyme-linked immunosorbent assay (ELISA), Direct Immuno enzymatic determination of thyrotropin/Hepatitis B in human serum through (ELISA) reader, Scientific approaches of ELISA and RIA technique.

(Available at- NOIDA, Mumbai, Chandigarh, Chennai, Hyderabad, Jaipur and Dehradun)

Applied Industrial Microbiology (Module 11)

Industrial microbiology application & major products, Major companies in India, Scope & job prospects, Good laboratory practices (GLP), Demonstration of module-oriented instrument, Different types of sterilization techniques in microbiology, Basic principle-Standard Operating Procedure (SOP) and Application of the instruments (Autoclave, pH meter, Laminar air flow, incubator, microscope and colony counter), Classification of bacteria based on Gram staining, Types of media and solution preparation, Basic calculation (Percentage, Molarity and Normality), Overview of serial dilution, Serial dilution of soil sample, Basic calculation of Colony Forming Unit (CFU)/ml, Screening of antibiotics producing microorganisms from soil samples, Identification of antibiotics producing microorganisms from soil samples using microbial staining methods, Biochemical assay for Bacteria (Amylase/Urease/Dextrose/Catalase/MR-VP), Different methods for the detection of antimicrobial activity, Antimicrobial activity of Antibiotic by disc diffusion method.

(Available at- NOIDA, Ahmedabad, Mumbai, Chandigarh, Chennai, Hyderabad, Jaipur, Dehradun and Gwalior)

Molecular Diagnostics and Laboratory Management Information System (Module 12)

Introduction to Clinical environment, Clinical sample collection and sample entry, Blood sample collection, Hospital Information Management System (HMIS)_features and functionalities, Sample storage and management, Differential staining of blood cells, Haemoglobin estimation from collected sample, Blood DNA isolation, DNA quantification by Gel electrophoresis, PCR Basics and concept, Molecular diagnostic PCR from isolated Blood DNA Sample, Results analysis and interpretation, Clinical Data Management-Basics, Advanced molecular diagnostics techniques: PCR and RT-PCR (T).

(Available at- NOIDA, Ahmedabad, Mumbai, Chandigarh, Chennai, Hyderabad, Jaipur and Dehradun)

Mathematics for Molecular Biologists and Medicine personnel (Module 13)

Introduction to Laboratory Math and its importance, Understanding metric systems and conversions, Molecular Calculations: Concentrations, Molarity and Normality), Buffer preparations, pH adjustments, Working with stock solutions, Culture media preparations: Cell Biology & Microbiology, Sterilization of Biosolutions, Storage and management of culture media, Handling of biological samples, Preparation of Standard curves for biological assays, Estimation of Proteins/ Carbohydrates/ Enzymes in samples, Result interpretations.

(Available at- NOIDA, Ahmedabad, Mumbai, Chandigarh, Chennai, Hyderabad, Jaipur and Dehradun)

Pharmaceutical Quality Control & Quality Assurance (Module 14)

Introduction and job prospects of pharmaceutical quality control. GLP in Pharmaceuticals, General and safety rules for working in the Lab, Demonstration of instruments, Clean room concept, Micropipette handling (forward and reverse pipetting techniques), Estimation of different tablet hardness through tablet hardness tester, Basic about the limit test in pharma, Limit test of heavy metal (lead), limit test of sulphate, limit test of chloride, Qualitative analysis of iodoform/oxalic acid/ester, Introduction about Minimum Inhibitory Concentration (MIC), MIC of antibiotics against pathogenic bacteria, Qualitative Assay of Acetaminophen/Acetylsalicylic Acid tablets, Principle-SOP and application of spectrophotometer, Percentage purity of Paracetamol from different brands of through UV-VIS spectrophotometer, Overview of the flame photometer, Analysis of different salts using a flame photometer, Principle-SOP and application of Karl Fischer Titration, Basic concept of Moisture analysis from different tablets through Karl Fischer Titration.

. (Available at- NOIDA, Mumbai, Chandigarh, Chennai, Hyderabad, Jaipur, Dehradun and Gwalior)

Juice and Soft drinks - Quality analysis & control skill (Module 15)

Introduction about juices and soft drinks, Types of beverages, General and safety rules for working in Lab, Micropipette handling (forward and reverse pipetting techniques), Demonstration of instruments, Classification of media used in microbiology, Basic concept of pH meter, Principle-Standard operating procedure and application of autoclave, Screening of microbial contamination and analysis of pH in different juices/soft drinks, Vitamin C content analysis in fresh juice sample via titration method, Acidity in different juices/soft drinks, Introduction and benefits of antioxidant, Estimation of antioxidant potential from different juices/soft drinks/non-alcoholic beverages, Principle-Standard operating procedure and application of Refractometer. Sugar content estimation through Refractometer, Quantitative estimation of proteins, Sodium benzoate analysis in soft drinks.

.(Available at- NOIDA, Ahmedabad, Mumbai, Chandigarh, Chennai, Hyderabad, Jaipur, Dehradun and Gwalior)

Dairy Technology- quality analysis and control skill (Module 16)

Introduction to dairy technology, Overview of Food Safety and Standards Authority of India (FSSAI).Concept of basic calculation (Percentage, Molarity and Normality) and reagent preparation, Micropipette handling (forward or reverse pipetting techniques), Standard operating procedure and application of hot air oven, Determination of Moisture from food samples, Acid value, fat content, coal tar dyes and total solid in milk/curd, Determination of moisture-fat and salt in butte, Iso-electric precipitation of Casei, Introduction and harmful effects of adulterant in milk, Adulterant analysis (Urea/Sulphate/Formalin/Hydrogen peroxide/Anionic Detergent, etc) in milk, Solubility percentage of milk powder, Methylene Blue Reductase Test (MBRT) in milk sample, Specific gravity test by lactometer /hydrometer, Introduction about saccharine, Adulteration of saccharine in different jam/ jelly/ Chewing gum/ice cream products.

(Available at- NOIDA, Ahmedabad, Mumbai, Chandigarh, Chennai, Hyderabad, Jaipur, Dehradun and Gwalior)

Probiotics Beverages Development (Module 17)

Introduction to probiotics and prebiotics, Benefits of probiotic bacteria to human health, Good Laboratory Practices (GLP), Micropipette handling (forward and reverse pipetting techniques. Basic instrument handling, Concept of basic calculation (Percentage, molarity, and normality), Overview of media preparation, Different types of sterilization in microbiology, Preparation of De Man, Rogosa, and Sharpe (MRS) media, Difference between spreading and streaking techniques, Isolation of probiotics bacteria from curd/yogurt/pickle via specialized enriched media, Bacteria classification according to gram staining, Characterization of probiotic isolates using Gram’s staining and Biochemical analysis (lactose/sucrose/dextrose/d-mannitol-acid secretion/ indole test/ protease test etc), Preparation of starter culture, Preparation of probiotic fruit beverage, Physicochemical analysis of probiotics health drink (pH, protein, and carbohydrate).

. (Available at- NOIDA, Ahmedabad, Mumbai, Chandigarh, Chennai, Hyderabad, Jaipur, Dehradun and Gwalior)

Formulations of Vegan Products and its sensory and nutritional evaluation (Module 18)

Introduction to Vegan concept, Basic concept of calculation, Micropipette handling (Reverse and Forward pipetting), Principle-Standard operating procedure and application of different instruments (Autoclave, Laminar air flow and pH meter), Vegan milk preparation (Soy/ Almond/ Groundnut/ Cashews/ Coconut), Vegan yogurt preparation: Selection of probiotic microbial species, Preparation of Inoculums, Product formulation and flavour selection, Sensory analysis of Vegan Yoghurt by 9-point Hedonic scale analysis, Nutritional analysis of Vegan Yoghurt (pH/ Acidity/ Moisture/ Total solids/ Carbohydrate/ Proteins, Quantitative analysis of Proteins or Carbohydrates in formulated Vegan products.

(Available at- NOIDA, Ahmedabad, Mumbai, Chandigarh, Chennai, Hyderabad, Jaipur, and Dehradun)

Bioprocess Engineering and Fermentation Technology (Module 19)

Introduction to Fermentation, Future Prospects in fermentation technology, Overview of upstream and downstream process, Precaution for maintenance and use of fermenter, General and safety rules for working in the Lab, Demonstration of instruments, Concept of basic calculation (Percentage, Molarity, and Normality), Micropipette handling (forward and reverse pipetting techniques), Overview of culture media preparation, Isolation and identification of citric acid-producing microorganisms (Bacteria/Fungi) from the soil sample, Preparation and sterilization of fermentation media, Operation process of Fermenter, Production of citric acid by submerged fermentation methods, Qualitative analysis of citric acid by titrimetric method, Basic concept of wine production parameter analysis by different physicochemical methods, Wine production from different fruit juices, Principle-standard operating procedure and application of spectrophotometer, Estimation of ethanol by potassium dichromate method through UV-VIS spectrophotometer, Standard Curve preparation and Data Interpretation.

. (Available at- NOIDA, Ahmedabad, Mumbai, Chandigarh, Chennai, Hyderabad, Jaipur and Dehradun)

Wastewater testing and Quality Analysis (Module 20)